Summary
12-O-Tetradecanoylphorbol-13-acetate (TPA) treatment induces human adenovirus (Ad) early region 1A (E1A) messenger ribonucleic acid expression in infected or Ad-transformed cells. Here, we report that deletion analysis has identified a TPA-responsive element (TRE) in the E1A enhancer region. Deletion analysis indicates that the TRE is located upstream of the E1A cap site between nucleotides −237 and −47. Incubation of extracts from TPA-treated cells with radioactively labeled deoxyribonucleic acid (DNA) fragments containing the TRE (−237 to −47) form specific DNA-protein complexes as demonstrated by gel shift analysis and Southwestern blotting. These experiments provide evidence that novel protein-DNA complexes are formed on a region of the E1A promoter required for TPA-enhanced expression. We speculate that these DNA-binding proteins may interact with the TRE and play a critical role in the mechanism through which TPA upregulates transcription from the Ad E1A gene.
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Gai, Q., James, C.B.L. Identification of a region on the adenovirus E1a gene responsible for induction by phorbol ester tumor promoter. In Vitro Cell.Dev.Biol.-Animal 37, 465–470 (2001). https://doi.org/10.1290/1071-2690(2001)037<0465:IOAROT>2.0.CO;2
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DOI: https://doi.org/10.1290/1071-2690(2001)037<0465:IOAROT>2.0.CO;2